CONTRASTING TOXICITY OF D-GALACTOSE ON OOCYTES AND EMBRYOS

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Vitrification of Oocytes and Embryos

Currently, controlled ovarian hyperstimulation protocols commonly provide embryos in excess of those needed for fresh transfer. Therefore, techniques have been developed to store these surplus embryos in liquid nitrogen (referred to as cryopreservation) for an indefinite period of time without significant compromise of their quality. Based on data from the Centers for Disease Control and Preven...

P-118: Developmental Competence of Ovine Embryos Derived from Frozen Oocytes Co-Cultured with Embryos Derived from Fresh Oocytes

Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...

Deleterious actions of gossypol on bovine spermatozoa, oocytes, and embryos.

Gossypol (50 and 100 micrograms/ml) decreased the percentage of sperm that completed the swim-up procedure. This effect was not blocked by glutathione monoethyl ester. Cleavage rates were not different between oocytes inseminated with gossypol-treated spermatozoa (10 or 50 micrograms/ml) and oocytes inseminated with control spermatozoa. Development to the blastocyst stage at Day 7 after insemin...

Quantitative microinjection of oocytes, eggs, and embryos.